Self-organization of "fibro-axonal" composite tissue around unmodified metallic micro-electrodes can form a functioning interface with a peripheral nerve: A new direction for creating long-term neural interfaces

Muscle Nerve. 2016 May;53(5):789-96. doi: 10.1002/mus.24928. Epub 2016 Jan 19.

Abstract

Introduction: A long-term peripheral neural interface is an area of intense research. The use of electrode interfaces is limited by the biological response to the electrode material.

Methods: We created an electrode construct to harbor the rat sciatic nerve with interposition of autogenous adipose tissue between the nerve and the electrode. The construct was implanted for 10 weeks.

Results: Immunohistochemistry showed a unique laminar pattern of axonal growth layered between fibro-collagenous tissue, forming a physical interface with the tungsten micro-electrode. Action potentials transmitted across the intrerface showed mean conduction velocities varying between 6.99 ± 2.46 and 20.14 ± 4 m/s.

Conclusions: We have demonstrated the feasibility of a novel peripheral nerve interface through modulation of normal biologic phenomena. It has potential applications as a chronic implantable neural interface.

Keywords: bionic; injury; interface; nerve; prosthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / physiology*
  • Adipose Tissue
  • Animals
  • Axons / pathology
  • Axons / physiology*
  • Collagen / physiology
  • Electrodes, Implanted*
  • Female
  • Immunohistochemistry
  • Metals
  • Microelectrodes*
  • Nerve Tissue / growth & development
  • Nerve Tissue / pathology
  • Nerve Tissue / physiology*
  • Neural Conduction / physiology*
  • Rats
  • Rats, Sprague-Dawley
  • Sciatic Nerve / growth & development
  • Sciatic Nerve / pathology
  • Sciatic Nerve / physiology*

Substances

  • Metals
  • Collagen