Abstract
Brain heart infusion agar containing 3 mg/liter vancomycin (BHI-V3) was used to screen for heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA). There was markedly greater biofilm formation by isolates that grew on BHI-V3 than by strains that did not grow on BHI-V3. Increased biofilm formation by hVISA may be mediated by FnbA- and polysaccharide intercellular adhesin-dependent pathways, and upregulation of atlA and sarA may also contribute to enhanced biofilm formation by hVISA upon prolonged exposure to vancomycin.
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MeSH terms
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Agar
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Biofilms / drug effects*
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Biofilms / growth & development
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Culture Media / chemistry
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Gene Expression Regulation, Bacterial*
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Genes, Bacterial*
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Humans
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Methicillin / pharmacology
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Methicillin-Resistant Staphylococcus aureus / drug effects*
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Methicillin-Resistant Staphylococcus aureus / genetics*
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Methicillin-Resistant Staphylococcus aureus / growth & development
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Methicillin-Resistant Staphylococcus aureus / metabolism
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Microbial Sensitivity Tests
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RNA, Messenger / genetics*
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RNA, Messenger / metabolism
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Staphylococcal Infections / microbiology
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Trans-Activators / genetics
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Trans-Activators / metabolism
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Vancomycin / pharmacology
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Vancomycin Resistance / genetics
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Culture Media
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RNA, Messenger
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SarA protein, bacterial
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Trans-Activators
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Vancomycin
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Agar
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Methicillin