Over-expression 5-aminolevulinic acid synthase 2 in nonerythroid cell may causes protoporphyrin IX accumulation

Hui Huang; Wei Wang; Jiaqi Zou; Zhiyan Liu; Zhongqiu Zhou; Osamu Nakajima; Lei Zhang; Jing Luo; Menghua Li; Qi He; Yan Diao; Huan Liang; Lingyun Zhou; Xu Gao

doi:10.1016/j.pdpdt.2015.10.004

Over-expression 5-aminolevulinic acid synthase 2 in nonerythroid cell may causes protoporphyrin IX accumulation

Photodiagnosis Photodyn Ther. 2015 Oct 12:S1572-1000(15)30036-3. doi: 10.1016/j.pdpdt.2015.10.004. Online ahead of print.

Authors

Hui Huang¹, Wei Wang¹, Jiaqi Zou¹, Zhiyan Liu¹, Zhongqiu Zhou¹, Osamu Nakajima², Lei Zhang¹, Jing Luo¹, Menghua Li¹, Qi He¹, Yan Diao¹, Huan Liang¹, Lingyun Zhou³, Xu Gao⁴

Affiliations

¹ Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China.
² Yamagata University, Japan.
³ Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China. Electronic address: [email protected].
⁴ Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China; Basic Medical Institute of Heilongjiang Medical Science Academy, Harbin, China. Electronic address: [email protected].

PMID: 26472512
DOI: 10.1016/j.pdpdt.2015.10.004

Abstract

Protoporphyrin IX (PpIX) has been used as an efficient sensitizer in photodynamic diagnose, photodynamic therapy, and sonodynamic therapy. The level of PpIX is very important for diagnose or therapy effects. 5-aminolevulinic acid synthase 2 (ALAS2) is the key enzyme upstream of PpIX synthesis. To increase PpIXaccumulation, ALAS2 overexppression transgenic mice were generated. Plasmid containing alas2 gene was transfected in colonic carcinoma cell lines. Both in tissues of transgenic mice and in colonic carcinoma cells, the amount of PpIXaccumulation did increased. At the meanwhile, level of heme, which down stream of PpIX had not been changed. Overexpression ALAS2 in nonerythriod cells may become a novel approach to cause PpIX accumulation.

Keywords: ALAS2; PpIX accumulation; nonerythriod; overexppression.