A trifunctional cyclooctyne for modifying azide-labeled biomolecules with photocrosslinking and affinity tags

Jessica A Stewart; Brent F Piligian; Sarah R Rundell; Benjamin M Swarts

doi:10.1039/c5cc07536j

A trifunctional cyclooctyne for modifying azide-labeled biomolecules with photocrosslinking and affinity tags

Chem Commun (Camb). 2015 Dec 25;51(99):17600-3. doi: 10.1039/c5cc07536j.

Authors

Jessica A Stewart¹, Brent F Piligian¹, Sarah R Rundell¹, Benjamin M Swarts¹

Affiliation

¹ Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA. [email protected].

PMID: 26482224
DOI: 10.1039/c5cc07536j

Abstract

A bicyclo[6.1.0]nonyne (BCN)-based cyclooctyne reagent bearing a photocrosslinking diazirine (DAz) group and a biotin affinity handle, BCN-DAz-Biotin, is reported. BCN-DAz-Biotin is capable of simultaneously delivering photocrosslinking and affinity tags to azide-labeled biomolecules, enabling photoactivated capture and enrichment/detection of interacting species in native contexts.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Affinity Labels*
Azides / chemistry*
Photochemical Processes*
Ultraviolet Rays

Substances

Affinity Labels
Azides