Abstract
We evaluated the analytical performance of a liquid chromatography-tandem mass spectrometry assay to detect carbapenemase activity in a group of carbapenemase-producing Enterobacteriaceae by meropenem hydrolysis. This one-hour method showed a sensitivity of 94% and a specificity of 100%, representing a rapid and reliable option compared to conventional phenotypic assays.
Keywords:
Carbapenem resistance; Carbapenemase activity; Enterobacteriaceae; Liquid chromatography-tandem mass spectrometry; Meropenem hydrolysis.
MeSH terms
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / metabolism*
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Bacterial Proteins / analysis
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Bacterial Proteins / chemistry
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Bacterial Proteins / metabolism*
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Biocatalysis
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Chromatography, Liquid / methods*
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Enterobacteriaceae / chemistry
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Enterobacteriaceae / enzymology*
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Enterobacteriaceae / isolation & purification
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Enterobacteriaceae / metabolism
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Enterobacteriaceae Infections / microbiology*
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Humans
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Meropenem
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Tandem Mass Spectrometry / methods*
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Thienamycins / chemistry
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Thienamycins / metabolism*
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beta-Lactamases / chemistry*
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beta-Lactamases / metabolism
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Thienamycins
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beta-Lactamases
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carbapenemase
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Meropenem