Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study

J Clin Microbiol. 2016 Jan;54(1):178-9. doi: 10.1128/JCM.01754-15. Epub 2015 Oct 21.

Abstract

We retrospectively evaluated the accuracy of conventional PCR targeting the 16S rRNA gene (16S C-PCR) using the Ot-16sRF1/Ot-16sRR1 primers for diagnosing scrub typhus. The diagnosis of Orientia tsutsugamushi infection by 16S C-PCR presented an increased sensitivity of 87.0% and specificity of 100% compared with those obtained with other targets and is thus a simple and clinically useful method with good diagnostic accuracy.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • DNA Primers / genetics*
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Orientia tsutsugamushi / genetics*
  • Polymerase Chain Reaction / methods*
  • RNA, Ribosomal, 16S / genetics*
  • Retrospective Studies
  • Scrub Typhus / diagnosis*
  • Sensitivity and Specificity

Substances

  • DNA Primers
  • RNA, Ribosomal, 16S

Grants and funding

This study was supported by a research fund from Chosun University, 2014.