Simple three-dimensional imaging of HRP-labelled neurons with the aid of an image processor

J Microsc. 1989 Feb;153(Pt 2):223-9. doi: 10.1111/j.1365-2818.1989.tb00562.x.

Abstract

A method is described for visualizing the three-dimensional structure of horseradish peroxidase-labelled neurons in the central nervous system of Xenopus laevis tadpoles. The reconstruction is simple, and performed on a commercially available image processing system. Minimal operational skill is required for using this method. Also, various improvements were made where user's simplicity (user-friendliness), speed and required computer memory are concerned. In addition, the excellent properties of the IBAS II system, such as image filtering and image editing functions are fully available, offering a fast, clean and complete final image reconstruction.

MeSH terms

  • Algorithms
  • Animals
  • Brain / cytology*
  • Computer Graphics
  • Computers
  • Horseradish Peroxidase
  • Image Processing, Computer-Assisted*
  • Immunoenzyme Techniques
  • Immunohistochemistry
  • Neurons / ultrastructure*
  • Xenopus laevis

Substances

  • Horseradish Peroxidase