Epigenomic Regulation of Smad1 Signaling During Cellular Senescence Induced by Ras Activation

Methods Mol Biol. 2016:1344:341-53. doi: 10.1007/978-1-4939-2966-5_22.

Abstract

Epigenomic modification plays important roles in regulating gene expression during development, differentiation, and cellular senescence. When oncogenes are activated, cells fall into stable growth arrest to block cellular proliferation, which is called oncogene-induced senescence. We recently identified through genome-wide analyses that Bmp2-Smad1 signal and its regulation by harmonized epigenomic alteration play an important role in Ras-induced senescence of mouse embryonic fibroblasts. We describe in this chapter the methods for analyses of epigenomic alteration and Smad1 targets on genome-wide scale.

Keywords: Bmp2; Chromatin immunoprecipitation (ChIP); Epigenetics; Histone; Smad1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Transformed
  • Cellular Senescence / physiology*
  • Chromatin Immunoprecipitation
  • Epigenesis, Genetic*
  • Epigenomics / methods
  • Fibroblasts
  • Gene Expression
  • Gene Expression Regulation*
  • Genes, Reporter
  • High-Throughput Nucleotide Sequencing
  • Histones / metabolism
  • Mice
  • Real-Time Polymerase Chain Reaction
  • Signal Transduction*
  • Smad1 Protein / metabolism*
  • Transcriptional Activation*
  • ras Proteins / genetics*

Substances

  • Histones
  • Smad1 Protein
  • ras Proteins