Double Copies of bla(KPC-3)::Tn4401a on an IncX3 Plasmid in Klebsiella pneumoniae Successful Clone ST512 from Italy

Daniela Fortini; Laura Villa; Claudia Feudi; João Pires; Celestino Bonura; Caterina Mammina; Andrea Endimiani; Alessandra Carattoli

doi:10.1128/AAC.01886-15

Double Copies of bla(KPC-3)::Tn4401a on an IncX3 Plasmid in Klebsiella pneumoniae Successful Clone ST512 from Italy

Antimicrob Agents Chemother. 2015 Nov 2;60(1):646-9. doi: 10.1128/AAC.01886-15. Print 2016 Jan.

Authors

Daniela Fortini¹, Laura Villa¹, Claudia Feudi¹, João Pires², Celestino Bonura³, Caterina Mammina³, Andrea Endimiani⁴, Alessandra Carattoli⁵

Affiliations

¹ Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy.
² Institute for Infectious Diseases, University of Bern, Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
³ Department of Sciences for Health Promotion and Mother-Child Care G. D'Alessandro, University of Palermo, Palermo, Italy.
⁴ Institute for Infectious Diseases, University of Bern, Bern, Switzerland.
⁵ Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy Institute for Infectious Diseases, University of Bern, Bern, Switzerland [email protected].

Abstract

A carbapenem-resistant sequence type 512 (ST512) Klebsiella pneumoniae carbapenemase 3 (KPC-3)-producing K. pneumoniae strain showing a novel variant plasmid content was isolated in Palermo, Italy, in 2014. ST512 is a worldwide successful clone associated with the spread of bla(KPC) genes located on the IncFIIk pKpQIL plasmid. In our ST512 strain, the bla(KPC-3) gene was unusually located on an IncX3 plasmid, whose complete sequence was determined. Two copies of bla(KPC-3)::Tn4401a caused by intramolecular transposition events were detected in the plasmid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Carbapenems / pharmacology
Clone Cells
DNA Transposable Elements*
Drug Resistance, Multiple, Bacterial / genetics*
Gene Dosage
Gene Expression Regulation, Bacterial*
Italy
Klebsiella Infections / microbiology
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / enzymology
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / isolation & purification
Microbial Sensitivity Tests
Open Reading Frames
Plasmids / chemistry*
Plasmids / metabolism
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Anti-Bacterial Agents
Bacterial Proteins
Carbapenems
DNA Transposable Elements
beta-Lactamases
carbapenemase

Associated data

GENBANK/KT362706

Grants and funding

The Italian Flagship “InterOmics” project (PB.P05) funded by MIUR and coordinated by the Italian Council of National Research (CNR) (A.C.) provided funding.