Intrauterine Bone Marrow Transplantation in Osteogenesis Imperfecta Mice Yields Donor Osteoclasts and Osteomacs but Not Osteoblasts

Susan M Millard; Allison R Pettit; Rebecca Ellis; Jerry K Y Chan; Liza J Raggatt; Kiarash Khosrotehrani; Nicholas M Fisk

doi:10.1016/j.stemcr.2015.09.017

Intrauterine Bone Marrow Transplantation in Osteogenesis Imperfecta Mice Yields Donor Osteoclasts and Osteomacs but Not Osteoblasts

Stem Cell Reports. 2015 Nov 10;5(5):682-689. doi: 10.1016/j.stemcr.2015.09.017. Epub 2015 Oct 29.

Authors

Susan M Millard¹, Allison R Pettit², Rebecca Ellis³, Jerry K Y Chan⁴, Liza J Raggatt², Kiarash Khosrotehrani⁵, Nicholas M Fisk⁶

Affiliations

¹ UQ Centre for Clinical Research, The University of Queensland, Herston, QLD 4029, Australia; Mater Research Institute, The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia. Electronic address: [email protected].
² UQ Centre for Clinical Research, The University of Queensland, Herston, QLD 4029, Australia; Mater Research Institute, The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia.
³ UQ Centre for Clinical Research, The University of Queensland, Herston, QLD 4029, Australia.
⁴ UQ Centre for Clinical Research, The University of Queensland, Herston, QLD 4029, Australia; Department of Reproductive Medicine, KK Women's and Children's Hospital, Singapore 229899, Singapore; Experimental Fetal Medicine Group, Department of Obstetrics and Gynaecology, National University of Singapore, Singapore 119228, Singapore.
⁵ UQ Centre for Clinical Research, The University of Queensland, Herston, QLD 4029, Australia; UQ Diamantina Institute, The University of Queensland, Translational Research Institute, Woolloongabba, QLD 4102, Australia.
⁶ UQ Centre for Clinical Research, The University of Queensland, Herston, QLD 4029, Australia; Royal Brisbane and Women's Hospital, Centre for Advanced Prenatal Care, Herston, QLD 4029, Australia.

Abstract

In this article, Millard and colleagues show that intrauterine bone marrow transplantation in the oim/oim mouse model of osteogenesis imperfecta yields hematopoietic microchimerism in the absence of donor osteopoiesis or phenotypic improvement. Bone-associated donor cells were not bone-forming osteoblasts, but osteoclasts (bone resorbing cells of the hematopoietic lineage) and osteal macrophages (bone regulatory cells of the hematopoietic lineage).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Marrow Cells / cytology*
Bone Marrow Transplantation*
Hematopoietic Stem Cell Transplantation*
Mice
Osteoblasts / cytology*
Osteoclasts / cytology*
Osteogenesis Imperfecta / surgery*