Abstract
Numerous plum pox virus (PPV) strain D isolates have been found in geographically distant regions of European Russia and the Crimean peninsula on different stone fruit hosts. Phylogenetic analysis of their partial and complete genomes suggests multiple introductions of PPV-D into Russia. Distinct natural isolates from Prunus tomentosa were found to bear unique amino acid substitutions in the N-terminus of the coat protein (CP) that may contribute to the adaptation of PPV-D to this host. Serological analysis using the PPV-D-specific monoclonal antibody 4DG5 provided further evidence that mutations at positions 58 and 59 of the CP are crucial for antibody binding.
Keywords:
454 Pyrosequencing; Phylogenetic analysis; Plum pox virus; Serological analysis; Strain D.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antibodies, Monoclonal / immunology
-
Antibodies, Viral / immunology
-
Capsid Proteins / genetics
-
Capsid Proteins / immunology
-
Cluster Analysis
-
Genetic Variation*
-
Genome, Viral
-
Molecular Sequence Data
-
Phylogeny
-
Plant Diseases / virology*
-
Plum Pox Virus / classification
-
Plum Pox Virus / genetics
-
Plum Pox Virus / immunology
-
Plum Pox Virus / isolation & purification*
-
Protein Binding
-
RNA, Viral / genetics
-
Russia
-
Sequence Analysis, DNA
-
Sequence Homology
Substances
-
Antibodies, Monoclonal
-
Antibodies, Viral
-
Capsid Proteins
-
RNA, Viral
Associated data
-
GENBANK/KP198583
-
GENBANK/KP198584
-
GENBANK/KP198585
-
GENBANK/KP198586
-
GENBANK/KP198587
-
GENBANK/KP198588
-
GENBANK/KP198589
-
GENBANK/KP198590
-
GENBANK/KP198591
-
GENBANK/KP198592
-
GENBANK/KP198593
-
GENBANK/KP198594
-
GENBANK/KP198595
-
GENBANK/KP198596
-
GENBANK/KP198597
-
GENBANK/KP198598
-
GENBANK/KP198599
-
GENBANK/KP198600
-
GENBANK/KP198601
-
GENBANK/KP198602
-
GENBANK/KR006729
-
GENBANK/KR006730
-
GENBANK/KR028385
-
GENBANK/KR028386
-
GENBANK/KR028387
-
GENBANK/KR028388
-
GENBANK/KR028389