A Betabaculovirus-Encoded gp64 Homolog Codes for a Functional Envelope Fusion Protein

Daniel M P Ardisson-Araújo; Fernando L Melo; Rollie J Clem; José L C Wolff; Bergmann M Ribeiro

doi:10.1128/JVI.02491-15

A Betabaculovirus-Encoded gp64 Homolog Codes for a Functional Envelope Fusion Protein

J Virol. 2015 Nov 4;90(3):1668-72. doi: 10.1128/JVI.02491-15. Print 2016 Feb 1.

Authors

Daniel M P Ardisson-Araújo¹, Fernando L Melo², Rollie J Clem³, José L C Wolff⁴, Bergmann M Ribeiro⁵

Affiliations

¹ Laboratory of Baculovirus, Cell Biology Department, University of Brasilia, Brasilia, DF, Brazil Division of Biology, Kansas State University, Manhattan, Kansas, USA.
² Laboratory of Baculovirus, Cell Biology Department, University of Brasilia, Brasilia, DF, Brazil.
³ Division of Biology, Kansas State University, Manhattan, Kansas, USA.
⁴ Laboratory of Molecular Biology and Virology, Biological Science and Health Center, Mackenzie Presbyterian University, Sao Paulo, SP, Brazil.
⁵ Laboratory of Baculovirus, Cell Biology Department, University of Brasilia, Brasilia, DF, Brazil [email protected].

Abstract

The GP64 envelope fusion protein is a hallmark of group I alphabaculoviruses. However, the Diatraea saccharalis granulovirus genome sequence revealed the first betabaculovirus species harboring a gp64 homolog (disa118). In this work, we have shown that this homolog encodes a functional envelope fusion protein and could enable the infection and fusogenic abilities of a gp64-null prototype baculovirus. Therefore, GP64 may complement or may be in the process of replacing F protein activity in this virus lineage.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Genetic Complementation Test
Granulovirus / genetics
Granulovirus / physiology*
Lepidoptera / virology
Viral Fusion Proteins / genetics*
Viral Fusion Proteins / metabolism*
Virus Internalization*

Substances

Viral Fusion Proteins