The complete mitochondrial DNA genome of a Chloridea (Heliothis) subflexa (Lepidoptera: Noctuidae) morpho-species

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4532-4533. doi: 10.3109/19401736.2015.1101549. Epub 2015 Nov 5.

Abstract

We investigate the complete mitogenome of a pheromone-trapped morpho-species of Chloridea subflexa from Brazil (initially identified by the Sanger sequencing of partial mtCOI gene) as 15 323 bp (KT598688) via next generation sequencing platform. The mitogenome has an A/T rich base composition (A: 40.4%; T: 40.3%; C: 11.5%; G: 7.8%), and included 13 protein-coding genes (PCGs), 22 tRNAs, 2 ribosomal RNAs and a putative replication region (ca. 323 bp). All PCGs start with a methionine (M) amino acid except the COI gene which has an arginine (R). The trnL2 and trn-Lys genes were partially embedded within the COII gene, while the trn-His gene was completely embedded within the ND4 gene. All PCGs ends with the "TAA" stop codon except ND3 which has a "TAG" stop codon.

Keywords: Biosecurity; heliothine moths; species genetic diversity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Composition
  • Codon, Terminator
  • DNA, Mitochondrial / chemistry
  • DNA, Mitochondrial / isolation & purification
  • DNA, Mitochondrial / metabolism
  • Electron Transport Complex IV / chemistry
  • Electron Transport Complex IV / genetics
  • Electron Transport Complex IV / metabolism
  • Genome, Mitochondrial
  • Insect Proteins / chemistry
  • Insect Proteins / genetics
  • Insect Proteins / metabolism
  • Moths / classification
  • Moths / genetics*
  • Open Reading Frames / genetics
  • Phylogeny
  • RNA, Ribosomal / chemistry
  • RNA, Ribosomal / genetics
  • RNA, Transfer / chemistry
  • RNA, Transfer / genetics
  • Sequence Analysis, DNA

Substances

  • Codon, Terminator
  • DNA, Mitochondrial
  • Insect Proteins
  • RNA, Ribosomal
  • RNA, Transfer
  • Electron Transport Complex IV