18F-THK5351: A Novel PET Radiotracer for Imaging Neurofibrillary Pathology in Alzheimer Disease

J Nucl Med. 2016 Feb;57(2):208-14. doi: 10.2967/jnumed.115.164848. Epub 2015 Nov 5.

Abstract

Imaging of neurofibrillary pathology in the brain helps in diagnosing dementia, tracking disease progression, and evaluating the therapeutic efficacy of antidementia drugs. The radiotracers used in this imaging must be highly sensitive and specific for tau protein fibrils in the human brain. We developed a novel tau PET tracer, (18)F-THK5351, through compound optimization of arylquinoline derivatives.

Methods: The in vitro binding properties, pharmacokinetics, and safety of (18)F-THK5351 were investigated, and a clinical study on Alzheimer disease (AD) patients was performed.

Results: (18)F-THK5351 demonstrated higher binding affinity for hippocampal homogenates from AD brains and faster dissociation from white-matter tissue than did (18)F-THK5117. The THK5351 binding amount correlated with the amount of tau deposits in human brain samples. Autoradiography of brain sections revealed that THK5351 bound to neurofibrillary tangles selectively and with a higher signal-to-background ratio than did THK5117. THK5351 exhibited favorable pharmacokinetics and no defluorination in mice. In first-in-human PET studies in AD patients, (18)F-THK5351 demonstrated faster kinetics, higher contrast, and lower retention in subcortical white matter than(18)F-THK5117.

Conclusion: (18)F-THK5351 is a useful PET tracer for the early detection of neurofibrillary pathology in AD patients.

Keywords: 18F-THK5351; Alzheimer’s disease; PET; radiotracer; tau.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / diagnostic imaging*
  • Alzheimer Disease / pathology
  • Aminopyridines / adverse effects
  • Aminopyridines / chemical synthesis
  • Aminopyridines / pharmacokinetics
  • Animals
  • Autoradiography
  • Biotransformation
  • Brain / diagnostic imaging
  • Brain / metabolism
  • Hippocampus / metabolism
  • Magnetic Resonance Imaging
  • Mice
  • Mice, Inbred ICR
  • Neurofibrillary Tangles / diagnostic imaging*
  • Neurofibrillary Tangles / pathology
  • Positron-Emission Tomography / methods*
  • Quinolines / adverse effects
  • Quinolines / chemical synthesis
  • Quinolines / pharmacokinetics
  • Radiation Dosage
  • Radiopharmaceuticals* / adverse effects
  • Radiopharmaceuticals* / chemical synthesis
  • Radiopharmaceuticals* / pharmacokinetics
  • Tissue Distribution
  • White Matter / diagnostic imaging
  • White Matter / metabolism
  • tau Proteins / metabolism

Substances

  • Aminopyridines
  • Quinolines
  • Radiopharmaceuticals
  • THK5351
  • tau Proteins