Mitochondrial targeting sequence variants of the CHCHD2 gene are a risk for Lewy body disorders

Neurology. 2015 Dec 8;85(23):2016-25. doi: 10.1212/WNL.0000000000002170. Epub 2015 Nov 11.

Abstract

Objective: To assess the role of CHCHD2 variants in patients with Parkinson disease (PD) and Lewy body disease (LBD) in Caucasian populations.

Methods: All exons of the CHCHD2 gene were sequenced in a US Caucasian patient-control series (878 PD, 610 LBD, and 717 controls). Subsequently, exons 1 and 2 were sequenced in an Irish series (355 PD and 365 controls) and a Polish series (394 PD and 350 controls). Immunohistochemistry and immunofluorescence studies were performed on pathologic LBD cases with rare CHCHD2 variants.

Results: We identified 9 rare exonic variants of unknown significance. These variants were more frequent in the combined group of PD and LBD patients compared to controls (0.6% vs 0.1%, p = 0.013). In addition, the presence of any rare variant was more common in patients with LBD (2.5% vs 1.0%, p = 0.050) compared to controls. Eight of these 9 variants were located within the gene's mitochondrial targeting sequence.

Conclusions: Although the role of variants of the CHCHD2 gene in PD and LBD remains to be further elucidated, the rare variants in the mitochondrial targeting sequence may be a risk factor for Lewy body disorders, which may link CHCHD2 to other genetic forms of parkinsonism with mitochondrial dysfunction.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Amino Acid Sequence
  • DNA-Binding Proteins
  • Female
  • Gene Targeting / methods*
  • Genetic Variation / genetics*
  • Humans
  • Lewy Body Disease / diagnosis
  • Lewy Body Disease / epidemiology
  • Lewy Body Disease / genetics*
  • Male
  • Middle Aged
  • Mitochondria / genetics*
  • Mitochondrial Proteins / genetics*
  • Molecular Sequence Data
  • Parkinson Disease / diagnosis
  • Parkinson Disease / epidemiology
  • Parkinson Disease / genetics*
  • Risk Factors
  • Transcription Factors / genetics*
  • Young Adult

Substances

  • CHCHD2 protein, human
  • DNA-Binding Proteins
  • Mitochondrial Proteins
  • Transcription Factors