Abstract
A semisynthetic gene precisely encoding the 502 amino acids of the human immunodeficiency virus type 1 gag precursor (Pr53gag) was expressed in the yeast Saccharomyces cerevisiae. Amino acid sequence analysis of the recombinant Pr53gag showed that the amino terminus was fully blocked. Labeling of Pr53gag with [3H]myristic acid demonstrated that, as with Pr53gag isolated from virus-infected cells, the yeast-derived protein was demethionylated and N myristylated on glycine, the second amino acid residue.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Gene Products, gag
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Genes*
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Genes, Synthetic*
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Genes, Viral*
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Genetic Vectors
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HIV-1 / enzymology
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HIV-1 / genetics*
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Myristic Acid
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Myristic Acids / metabolism*
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Protein Processing, Post-Translational*
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Protein-Tyrosine Kinases / genetics*
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Restriction Mapping
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Retroviridae Proteins / genetics*
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Saccharomyces cerevisiae / genetics*
Substances
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Gene Products, gag
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Myristic Acids
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Retroviridae Proteins
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Myristic Acid
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Protein-Tyrosine Kinases