Angiotensin II receptor blockade promotes repair of skeletal muscle through down-regulation of aging-promoting C1q expression

Sci Rep. 2015 Sep 25:5:14453. doi: 10.1038/srep14453.

Abstract

Disruption of angiotensin II type 1 (AT1) receptor prolonged life span in mice. Since aging-related decline in skeletal muscle function was retarded in Atgr1a(-/-) mice, we examined the role of AT1 receptor in muscle regeneration after injury. Administration of AT1 receptor blocker irbesartan increased the size of regenerating myofibers, decreased fibrosis, and enhanced functional muscle recovery after cryoinjury. We recently reported that complement C1q, secreted by macrophages, activated Wnt/β-catenin signaling and promoted aging-related decline in regenerative capacity of skeletal muscle. Notably, irbesartan induced M2 polarization of macrophages, but reduced C1q expression in cryoinjured muscles and in cultured macrophage cells. Irbesartan inhibited up-regulation of Axin2, a downstream gene of Wnt/β-catenin pathway, in cryoinjured muscles. In addition, topical administration of C1q reversed beneficial effects of irbesartan on skeletal muscle regeneration after injury. These results suggest that AT1 receptor blockade improves muscle repair and regeneration through down-regulation of the aging-promoting C1q-Wnt/β-catenin signaling pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Topical
  • Aging / genetics
  • Angiotensin II Type 1 Receptor Blockers / pharmacology*
  • Animals
  • Axin Protein / metabolism
  • Biphenyl Compounds / pharmacology
  • Cell Line
  • Complement C1q / genetics*
  • Complement C1q / metabolism
  • Down-Regulation / drug effects*
  • Immunohistochemistry
  • Irbesartan
  • Macrophages / cytology
  • Macrophages / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Muscle, Skeletal / metabolism*
  • Muscle, Skeletal / pathology
  • PAX7 Transcription Factor / metabolism
  • Receptor, Angiotensin, Type 1 / chemistry
  • Receptor, Angiotensin, Type 1 / genetics
  • Receptor, Angiotensin, Type 1 / metabolism*
  • Regeneration / physiology
  • Tetrazoles / pharmacology
  • Wnt Signaling Pathway / drug effects

Substances

  • Angiotensin II Type 1 Receptor Blockers
  • Axin Protein
  • Axin2 protein, mouse
  • Biphenyl Compounds
  • PAX7 Transcription Factor
  • Pax7 protein, mouse
  • Receptor, Angiotensin, Type 1
  • Tetrazoles
  • Complement C1q
  • Irbesartan