Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

Chem Commun (Camb). 2016 Jan 18;52(5):954-7. doi: 10.1039/c5cc09131d.

Abstract

A cleavable azo linker was synthesized and reacted with 5-(6)-carboxytetramethyl rhodamine succinimidyl ester, followed by further reactions with di(N-succinimidyl) carbonate and 5-(3-amino-1-propynyl)-2'-deoxyuridine 5'-triphosphate [dUTP(AP3)] to obtain the terminal product dUTP-azo linker-TAMRA as a potential reversible terminator for DNA sequencing by synthesis with no need for 3'-OH blocking.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azo Compounds / chemistry*
  • Drug Design*
  • Fluorescence*
  • Molecular Structure
  • Nucleotides / chemical synthesis*
  • Nucleotides / chemistry*
  • Sequence Analysis, DNA / methods*

Substances

  • Azo Compounds
  • Nucleotides