Increased levels of etheno-DNA adducts and genotoxicity biomarkers of long-term exposure to pure diesel engine exhaust

Meili Shen; Ping Bin; Haibin Li; Xiao Zhang; Xin Sun; Huawei Duan; Yong Niu; Tao Meng; Yufei Dai; Weimin Gao; Shanfa Yu; Guizhen Gu; Yuxin Zheng

doi:10.1016/j.scitotenv.2015.10.165

Increased levels of etheno-DNA adducts and genotoxicity biomarkers of long-term exposure to pure diesel engine exhaust

Sci Total Environ. 2016 Feb 1;543(Pt A):267-273. doi: 10.1016/j.scitotenv.2015.10.165. Epub 2015 Nov 14.

Authors

Meili Shen¹, Ping Bin², Haibin Li³, Xiao Zhang⁴, Xin Sun⁵, Huawei Duan⁶, Yong Niu⁷, Tao Meng⁸, Yufei Dai⁹, Weimin Gao¹⁰, Shanfa Yu¹¹, Guizhen Gu¹², Yuxin Zheng¹³

Affiliations

¹ Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. Electronic address: [email protected].
² Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. Electronic address: [email protected].
³ Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. Electronic address: [email protected].
⁴ Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. Electronic address: [email protected].
⁵ Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. Electronic address: [email protected].
⁶ Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. Electronic address: [email protected].
⁷ Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. Electronic address: [email protected].
⁸ Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. Electronic address: [email protected].
⁹ Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. Electronic address: [email protected].
¹⁰ Department of Environmental Toxicology, The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79409, USA. Electronic address: [email protected].
¹¹ Henan Provincial Institute for Occupational Health, Zhengzhou, 450052, China. Electronic address: [email protected].
¹² Henan Provincial Institute for Occupational Health, Zhengzhou, 450052, China. Electronic address: [email protected].
¹³ Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. Electronic address: [email protected].

PMID: 26588802
DOI: 10.1016/j.scitotenv.2015.10.165

Abstract

Etheno-DNA adducts are biomarkers for assessing oxidative stress. In this study, the aim was to detect the level of etheno-DNA adducts and explore the relationship between the etheno-DNA adducts and genotoxicity biomarkers of the diesel engine exhaust (DEE)-exposed workers. We recruited 86 diesel engine testing workers with long-term exposure to DEE and 99 non-DEE-exposed workers. The urinary mono-hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) and etheno-DNA adducts (εdA and εdC) were detected by HPLC-MS/MS and UPLC-MS/MS, respectively. Genotoxicity biomarkers were also evaluated by comet assay and cytokinesis-block micronucleus assay. The results showed that urinary εdA was significantly higher in the DEE-exposed workers (p<0.001), exhibited 2.1-fold increase compared with the non-DEE-exposed workers. The levels of urinary OH-PAHs were positively correlated with the level of εdA among all the study subjects (p<0.001). Moreover, we found that the increasing level of εdA was significantly associated with the increased olive tail moment, percentage of tail DNA, or frequency of micronucleus in the study subjects (p<0.01). No significant association was observed between the εdC level and any measured genotoxicity biomarkers. In summary, εdA could serve as an indicator for DEE exposure in the human population.

Keywords: Comet assay; Diesel engine exhaust; Etheno-DNA adducts; Micronucleus; OH-PAHs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Air Pollutants / analysis
Air Pollutants / toxicity*
Air Pollution / statistics & numerical data
Biomarkers / metabolism
Chromatography, High Pressure Liquid
Comet Assay
DNA
DNA Adducts
DNA Damage
Female
Humans
Inhalation Exposure / analysis
Inhalation Exposure / statistics & numerical data*
Male
Micronucleus Tests
Occupational Exposure / analysis
Occupational Exposure / statistics & numerical data*
Oxidative Stress
Polycyclic Aromatic Hydrocarbons / analysis
Polycyclic Aromatic Hydrocarbons / toxicity
Vehicle Emissions / analysis
Vehicle Emissions / toxicity*

Substances

Air Pollutants
Biomarkers
DNA Adducts
Polycyclic Aromatic Hydrocarbons
Vehicle Emissions
DNA