Acclimation of a marine microbial consortium for efficient Mn(II) oxidation and manganese containing particle production

J Hazard Mater. 2016 Mar 5:304:434-40. doi: 10.1016/j.jhazmat.2015.11.019. Epub 2015 Nov 30.

Abstract

Sediment contamination with metals is a widespread concern in the marine environment. Manganese oxidizing bacteria (MOB) are extensively distributed in various environments, but a marine microbial community containing MOB is rarely reported. In this study, a consortium of marine metal-contaminated sediments was acclimated using Mn(II). The shift in community structure was determined through high-throughput sequencing. In addition, the consortium resisted several harsh conditions, such as toxic metals (1mM Cu(II) and Fe(III)), and exhibited high Mn(II) oxidation capacities even the Mn(II) concentration was up to 5mM. Meanwhile, biogenic Mn containing particles were characterized by scanning electron microscope (SEM), X-ray powder diffraction (XRD), and N2 adsorption/desorption. Dye removal performance of the Mn containing particles was assayed using methylene blue, and 20.8 mg g(-1) adsorption capacity was obtained. Overall, this study revealed several new genera associated with Mn(II) oxidation and rare biogenic Na3MnPO4CO3. Results suggested the complexity of natural microbe-mediated Mn transformation.

Keywords: Bioremediation; High-throughput sequencing; Manganese oxidizing bacteria; Microbial consortium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Bacteria / genetics
  • Bacteria / isolation & purification
  • Bacteria / metabolism
  • Coloring Agents / chemistry
  • DNA, Bacterial / analysis
  • Geologic Sediments / microbiology*
  • Manganese / chemistry
  • Manganese / metabolism*
  • Metal Nanoparticles* / chemistry
  • Metal Nanoparticles* / ultrastructure
  • Methylene Blue / chemistry
  • Microbial Consortia
  • Microscopy, Electron, Scanning
  • Oxidation-Reduction
  • Seawater / microbiology

Substances

  • Coloring Agents
  • DNA, Bacterial
  • Manganese
  • Methylene Blue