Mesenchymal-epithelial interaction techniques

Gerald R Cunha; Lawrence Baskin

doi:10.1016/j.diff.2015.10.006

Mesenchymal-epithelial interaction techniques

Differentiation. 2016 Apr-Jun;91(4-5):20-7. doi: 10.1016/j.diff.2015.10.006. Epub 2015 Nov 21.

Authors

Gerald R Cunha¹, Lawrence Baskin²

Affiliations

¹ Department of Urology, University of California, Box 0738, San Francisco, CA 94143, United States. Electronic address: [email protected].
² Department of Urology, University of California, Box 0738, San Francisco, CA 94143, United States.

Abstract

This paper reviews the importance of mesenchymal-epithelial interactions in development and gives detailed technical protocols for investigating these interactions. Successful analysis of mesenchymal-epithelial interactions requires knowing the ages in which embryonic, neonatal and adult organs can be separated into mesenchymal and epithelial tissues. Methods for separation of mesenchymal and epithelial tissues and preparation of tissue recombinants are described.

Keywords: Development; Differentiation; Epithelium; Mesenchymal-epithelial interactions; Mesenchyme; Tissue recombinants.

Publication types

Review
Research Support, N.I.H., Extramural

MeSH terms

Animals
Cell Differentiation / genetics*
Cellular Reprogramming / genetics
Epithelium / growth & development*
Humans
Mesoderm / growth & development*
Mice
Organogenesis / genetics

Grants and funding

R01 DK058105/DK/NIDDK NIH HHS/United States