Abstract
In vitro follicle growth is a potential approach to preserve fertility for young women who are facing a risk of premature ovarian failure (POF) caused by radiation or chemotherapy. Our two-step follicle culture strategy recapitulated the dynamic human follicle growth environment in vitro. Follicles developed from the preantral to antral stage, and, for the first time, produced meiotically competent metaphase II (MII) oocytes after in vitro maturation (IVM).
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Adult
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Alginates / chemistry
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Anti-Mullerian Hormone / biosynthesis
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Anti-Mullerian Hormone / metabolism
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Estradiol / biosynthesis
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Estradiol / metabolism
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Female
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Fertilization in Vitro*
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Glucuronic Acid / chemistry
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Hexuronic Acids / chemistry
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Humans
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Hydrogels / chemistry
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Metaphase
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Oocytes / cytology
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Oocytes / growth & development*
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Oocytes / metabolism
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Ovarian Follicle / cytology
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Ovarian Follicle / growth & development*
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Ovarian Follicle / metabolism
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Primary Ovarian Insufficiency / prevention & control
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Progesterone / biosynthesis
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Progesterone / metabolism
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Tissue Culture Techniques / instrumentation
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Tissue Culture Techniques / methods*
Substances
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Alginates
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Hexuronic Acids
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Hydrogels
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Progesterone
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Estradiol
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Anti-Mullerian Hormone
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Glucuronic Acid