Nicotiana benthamiana has shown great success as a platform for the production of recombinant proteins. Here, we describe methods to transiently express high levels of recombinant proteins and simultaneously modulate their glycosylation pattern toward human-like structures. The method aims to generate recombinant proteins with a targeted largely homogeneous glycosylation profile for structure-function studies.
Keywords: Glycoengineering; Nicotiana benthamiana; Recombinant proteins; Transient expression.