An Ex vivo culture model for placental cytomegalovirus infection using slices of Guinea pig placental tissue

Souichi Yamada; Harutaka Katano; Yuko Sato; Saki Fukuchi; Kaede Hashimoto; Naoki Inoue

doi:10.1016/j.placenta.2015.10.016

An Ex vivo culture model for placental cytomegalovirus infection using slices of Guinea pig placental tissue

Placenta. 2016 Jan:37:85-8. doi: 10.1016/j.placenta.2015.10.016. Epub 2015 Nov 18.

Authors

Souichi Yamada¹, Harutaka Katano², Yuko Sato², Saki Fukuchi³, Kaede Hashimoto³, Naoki Inoue⁴

Affiliations

¹ Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan. Electronic address: [email protected].
² Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
³ Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan.
⁴ Department of Virology I, National Institute of Infectious Diseases, Tokyo, Japan; Department of Microbiology and Immunology, Gifu Pharmaceutical University, Gifu, Japan. Electronic address: [email protected].

PMID: 26625961
DOI: 10.1016/j.placenta.2015.10.016

Abstract

Congenital infection with human cytomegalovirus (CMV) through the placenta is one of the major causes of birth and developmental abnormalities. Guinea pig CMV (GPCMV) causes in utero infection, which makes its animal models useful for studies on congenital diseases. Here, we established an ex vivo culture method for tissue slices prepared from guinea pig placentas and demonstrated that viral spread in the model resembles those in the placenta of GPCMV-infected animals and that the infection is independent of the pentameric glycoprotein complex for endothelial/epithelial cell tropism. Thus, this model affords a useful tool for pathobiological studies on CMV placental infection.

Keywords: Congenital infection; Cytomegalovirus; Guinea pig; Placenta; Slice culture.

MeSH terms

Animals
Cell Culture Techniques / methods*
Cytomegalovirus Infections / congenital*
Cytomegalovirus Infections / pathology*
Disease Models, Animal
Female
Guinea Pigs
Infectious Disease Transmission, Vertical
Models, Theoretical
Organ Culture Techniques
Placenta / pathology*
Placenta / virology
Pregnancy
Pregnancy Complications, Infectious / pathology*
Primary Cell Culture / methods