Abstract
Organometallic complexes with thiopyrone-based ligands have shown promising cytotoxic activity in vitro. To investigate the impact of the ligand backbone modification of these biologically active compounds and enhance the solubility in aqueous solution, the (thio)pyrone scaffold was modified via Suzuki-Miyaura coupling reaction and converted into corresponding organometallic Ru(ii) and Rh(iii) complexes. Characterization of the synthesized compounds was carried out by means of 1D and 2D NMR, ESI MS, and also by X-ray diffraction analysis. The stability in aqueous solution was investigated via(1)H NMR spectroscopy. Due to the close structural resemblance to flavonoids, topoisomerase inhibition, the cytotoxicity in human cancer cell lines as well as ROS generation was investigated by means of the topoisomerase II drug screening assay, the MTT assay and DCFH-DA assay, respectively.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antigens, Neoplasm / metabolism
-
Antineoplastic Agents / chemical synthesis
-
Antineoplastic Agents / chemistry
-
Antineoplastic Agents / pharmacology
-
Cell Line, Tumor
-
Cell Survival / drug effects
-
Coordination Complexes / chemical synthesis
-
Coordination Complexes / chemistry*
-
Coordination Complexes / pharmacology
-
Crystallography, X-Ray
-
DNA Topoisomerases, Type II / metabolism
-
DNA-Binding Proteins / antagonists & inhibitors
-
DNA-Binding Proteins / metabolism
-
Drug Screening Assays, Antitumor
-
Humans
-
Ligands
-
Molecular Conformation
-
Organometallic Compounds / chemistry*
-
Pyrones / chemistry*
-
Reactive Oxygen Species / metabolism
-
Ruthenium / chemistry
-
Solubility
-
Structure-Activity Relationship
Substances
-
Antigens, Neoplasm
-
Antineoplastic Agents
-
Coordination Complexes
-
DNA-Binding Proteins
-
Ligands
-
Organometallic Compounds
-
Pyrones
-
Reactive Oxygen Species
-
Ruthenium
-
DNA Topoisomerases, Type II