Vaspin prevents elevation of blood pressure through inhibition of peripheral vascular remodelling in spontaneously hypertensive rats

Acta Physiol (Oxf). 2016 Jun;217(2):120-9. doi: 10.1111/apha.12636. Epub 2015 Dec 24.

Abstract

Aim: Visceral adipose tissue-derived serine protease inhibitor (vaspin) is a relatively novel adipocytokine with protective effects on metabolic diseases including obesity and type II diabetes. We have previously demonstrated that vaspin exerts anti-inflammatory and antimigratory roles through antioxidative effects in vascular smooth muscle cells. As inflammatory responses and migration of smooth muscle in peripheral vascular wall are key mechanisms for the pathogenesis of hypertension, we hypothesized that vaspin could prevent the development of hypertension in in vivo hypertensive animal model.

Methods: Vaspin (1 μg kg(-1) day(-1) ) was administered intraperitoneally to 5-week-old male spontaneously hypertensive rats (SHR) for 4 weeks. Superior mesenteric artery was isolated and used for measurement of isometric contraction and histological analysis.

Results: Long-term vaspin treatment significantly prevented an elevation of systolic blood pressure (SBP) at 8 weeks of age. Vaspin had no effect on reactivity of isolated mesenteric artery from SHR. In contrast, vaspin significantly inhibited mesenteric arterial wall hypertrophy in SHR. Moreover, vaspin significantly inhibited an increase of tumour necrosis factor-α expression and a production of reactive oxygen species in isolated mesenteric artery from SHR.

Conclusion: This study for the first time demonstrates that vaspin prevents the increase of SBP in SHR through inhibiting peripheral vascular hypertrophy possibly via antioxidative and anti-inflammatory mechanisms.

Keywords: adipokine; arterial wall remodeling; hypertension; inflammation; oxidative stress.

MeSH terms

  • Animals
  • Antihypertensive Agents / administration & dosage
  • Blood Pressure / drug effects*
  • Dose-Response Relationship, Drug
  • Hypertension / drug therapy*
  • Hypertension / physiopathology*
  • Infusions, Parenteral
  • Male
  • Mesenteric Artery, Superior / drug effects
  • Mesenteric Artery, Superior / physiopathology*
  • Rats
  • Rats, Inbred SHR
  • Serpins / administration & dosage*
  • Treatment Outcome
  • Vascular Remodeling / drug effects*
  • Vascular Resistance / drug effects

Substances

  • Antihypertensive Agents
  • Serpina12 protein, rat
  • Serpins