Multiplexed Activity of perAuxidase: DNA-Capped AuNPs Act as Adjustable Peroxidase

Mustafa Salih Hizir; Meryem Top; Mustafa Balcioglu; Muhit Rana; Neil M Robertson; Fusheng Shen; Jia Sheng; Mehmet V Yigit

doi:10.1021/acs.analchem.5b03926

Multiplexed Activity of perAuxidase: DNA-Capped AuNPs Act as Adjustable Peroxidase

Anal Chem. 2016 Jan 5;88(1):600-5. doi: 10.1021/acs.analchem.5b03926. Epub 2015 Dec 15.

Authors

Mustafa Salih Hizir¹, Meryem Top¹, Mustafa Balcioglu¹, Muhit Rana¹, Neil M Robertson¹, Fusheng Shen¹, Jia Sheng^{1

2}, Mehmet V Yigit^{1

2}

Affiliations

¹ Department of Chemistry, University at Albany, State University of New York , 1400 Washington Avenue, Albany, New York 12222, United States.
² The RNA Institute, University at Albany, State University of New York , 1400 Washington Avenue, Albany, New York 12222, United States.

PMID: 26654642
DOI: 10.1021/acs.analchem.5b03926

Abstract

In this study, we have investigated the intrinsic peroxidase-like activity of citrate-capped AuNPs (perAuxidase) and demonstrated that the nanozyme function can be multiplexed and tuned by integrating oligonucleotides on a nanoparticle surface. Systematic studies revealed that by controlling the reaction parameters, the mutiplexing effect can be delayed or advanced and further used for aptasensor applications.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Benzidines / chemistry
Benzidines / metabolism
DNA / chemistry
DNA / metabolism*
Gold / chemistry
Gold / metabolism*
Hydrogen Peroxide / chemistry
Hydrogen Peroxide / metabolism*
Metal Nanoparticles / chemistry*
Peroxidase / chemistry
Peroxidase / metabolism*
Surface Properties

Substances

Benzidines
3,3',5,5'-tetramethylbenzidine
Gold
DNA
Hydrogen Peroxide
Peroxidase