Formation and Characterization of Supported Lipid Bilayers Composed of Hydrogenated and Deuterated Escherichia coli Lipids

Tania Kjellerup Lind; Hanna Wacklin; Jürgen Schiller; Martine Moulin; Michael Haertlein; Thomas Günther Pomorski; Marité Cárdenas

doi:10.1371/journal.pone.0144671

Formation and Characterization of Supported Lipid Bilayers Composed of Hydrogenated and Deuterated Escherichia coli Lipids

PLoS One. 2015 Dec 11;10(12):e0144671. doi: 10.1371/journal.pone.0144671. eCollection 2015.

Authors

Tania Kjellerup Lind^{1

2}, Hanna Wacklin^{1

2}, Jürgen Schiller³, Martine Moulin⁴, Michael Haertlein⁴, Thomas Günther Pomorski⁵, Marité Cárdenas^{1

6}

Affiliations

¹ Nano-Science Center and Department of Chemistry, University of Copenhagen, Copenhagen, Denmark.
² European Spallation Source ESS AB, Lund, Sweden.
³ Institute of Medical Physics and Biophysics, Faculty of Medicine, University of Leipzig, Leipzig, Germany.
⁴ Institut Laue-Langevin, Life Science Group, Grenoble, France.
⁵ Centre for Membrane Pumps in Cells and Disease-PUMPKIN, Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen, Denmark.
⁶ Malmoe University, Department of Biomedical Sciences, Health & Society, 20500 Malmoe, Sweden.

Abstract

Supported lipid bilayers are widely used for sensing and deciphering biomolecular interactions with model cell membranes. In this paper, we present a method to form supported lipid bilayers from total lipid extracts of Escherichia coli by vesicle fusion. We show the validity of this method for different types of extracts including those from deuterated biomass using a combination of complementary surface sensitive techniques; quartz crystal microbalance, neutron reflection and atomic force microscopy. We find that the head group composition of the deuterated and the hydrogenated lipid extracts is similar (approximately 75% phosphatidylethanolamine, 13% phosphatidylglycerol and 12% cardiolipin) and that both samples can be used to reconstitute high-coverage supported lipid bilayers with a total thickness of 41 ± 3 Å, common for fluid membranes. The formation of supported lipid bilayers composed of natural extracts of Escherichia coli allow for following biomolecular interactions, thus advancing the field towards bacterial-specific membrane biomimics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cardiolipins / chemistry
Deuterium / chemistry*
Escherichia coli / chemistry*
Hydrogen / chemistry*
Hydrogenation
Kinetics
Lipid Bilayers / chemistry*
Membrane Fusion
Microscopy, Atomic Force
Phosphatidylethanolamines / chemistry
Phosphatidylglycerols / chemistry
Quartz Crystal Microbalance Techniques
Thermodynamics

Substances

Cardiolipins
Lipid Bilayers
Phosphatidylethanolamines
Phosphatidylglycerols
phosphatidylethanolamine
Hydrogen
Deuterium

Grants and funding

The work was supported by the following: European Spallation Source ESS AB in Lund, Sweden (TKL); Danscatt Centre funded by the Danish government (MC); The Research Centre 'bioSYNergy' at University of Copenhagen funded by the UCPH Excellence Program for Interdisciplinary Research (TGP and MC); The Swedish Research Council (MC); and The Engineering and Physical Sciences Research Council (grants GR/R99393/01 and EP/C015452/1), EU contracts RII3-CT-2003-505925 and NMP4-CT-2006-033256 (MH). The European Spallation Source provided support in the form of salaries for author Tania K. Lind, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of this author are articulated in the "author contributions" section.