On the mineral core of ferritin-like proteins: structural and magnetic characterization

Nanoscale. 2016 Jan 14;8(2):1088-99. doi: 10.1039/c5nr04446d.

Abstract

It is generally accepted that the mineral core synthesized by ferritin-like proteins consists of a ferric oxy-hydroxide mineral similar to ferrihydrite in the case of horse spleen ferritin (HoSF) and an oxy-hydroxide-phosphate phase in plant and prokaryotic ferritins. The structure reflects a dynamic process of deposition and dissolution, influenced by different biological, chemical and physical variables. In this work we shed light on this matter by combining a structural (High Resolution Transmission Electron Microscopy (HRTEM) and Fe K-edge X-ray Absorption Spectroscopy (XAS)) and a magnetic study of the mineral core biomineralized by horse spleen ferritin (HoSF) and three prokaryotic ferritin-like proteins: bacterial ferritin (FtnA) and bacterioferritin (Bfr) from Escherichia coli and archaeal ferritin (PfFtn) from Pyrococcus furiosus. The prokaryotic ferritin-like proteins have been studied under native conditions and inside the cells for the sake of preserving their natural attributes. They share with HoSF a nanocrystalline structure rather than an amorphous one as has been frequently reported. However, the presence of phosphorus changes drastically the short-range order and magnetic response of the prokaryotic cores with respect to HoSF. The superparamagnetism observed in HoSF is absent in the prokaryotic proteins, which show a pure atomic-like paramagnetic behaviour attributed to phosphorus breaking the Fe-Fe exchange interaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / chemistry
  • Cytochrome b Group / chemistry
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / chemistry
  • Ferritins / chemistry*
  • Horses
  • Hydroxides / chemistry
  • Iron / chemistry
  • Magnetics*
  • Microscopy, Electron, Transmission
  • Nanoparticles
  • Nanotechnology / methods*
  • Phosphorus / chemistry
  • Pyrococcus furiosus / chemistry
  • Recombinant Proteins / chemistry
  • Spectrophotometry
  • Spleen / chemistry

Substances

  • Bacterial Proteins
  • Cytochrome b Group
  • Hydroxides
  • Recombinant Proteins
  • Phosphorus
  • Ferritins
  • bacterioferritin
  • Iron