Abstract
The analysis of gene expression in cytotoxic T cells by in situ hybridization of serial liver and brain sections from mice infected with lymphocytic choriomeningitis virus (LCMV) and immunostaining with T cell marker- and virus-specific antibodies revealed a close histological association of infiltrating lymphocytes expressing the perforin and granzyme A genes with virally infected cells. Maximal frequency of perforin and granzyme A mRNA-containing cells on liver sections preceded by about 2 days maximal LCMV-specific cytotoxicity of the lymphoid liver infiltrating cells. These results are most consistent with an involvement of perforin and granzyme A in cell-mediated cytotoxicity in vivo.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Brain / microbiology
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Brain / physiopathology
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Gene Expression Regulation
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Granzymes
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Hepatitis, Viral, Animal / genetics
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Immunoenzyme Techniques
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Liver / microbiology
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Liver / pathology
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Liver / physiopathology
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Lymphocytic Choriomeningitis / microbiology
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Lymphocytic Choriomeningitis / pathology
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Lymphocytic Choriomeningitis / physiopathology*
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Membrane Glycoproteins*
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Membrane Proteins / genetics*
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Mice
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Nucleic Acid Hybridization
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Perforin
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Pore Forming Cytotoxic Proteins
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RNA Probes
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RNA, Messenger / genetics
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Serine Endopeptidases / genetics*
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T-Lymphocytes, Cytotoxic / physiology*
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Time Factors
Substances
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Membrane Glycoproteins
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Membrane Proteins
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Pore Forming Cytotoxic Proteins
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RNA Probes
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RNA, Messenger
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Perforin
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Granzymes
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Serine Endopeptidases