The Generally Useful Estimate of Solvent Systems (GUESS) method enables the rapid purification of methylpyridoxine regioisomers by countercurrent chromatography

J Chromatogr A. 2015 Dec 24:1426:248-51. doi: 10.1016/j.chroma.2015.11.046. Epub 2015 Nov 17.

Abstract

The TLC-based Generally Useful Estimate of Solvent Systems (GUESS) method was employed for countercurrent chromatography solvent system selection, in order to separate the three synthetic isomers: 3-O-methylpyridoxine, 4'-O-methylpyridoxine (ginkgotoxin), and 5'-O-methylpyridoxine. The Rf values of the three isomers indicated that ChMWat+2 (chloroform-methanol-water 10:5:5, v/v/v) was appropriate for the countercurrent separation. The isomer separation was highly selective and demonstrated that the TLC-based GUESS method can accelerate solvent system selection for countercurrent separation. Accordingly, the study re-emphasizes the practicality of TLC as a tool to facilitate the rapid development of new countercurrent and centrifugal partition chromatography methods for this solvent system. Purity and structure characterization of all samples was performed by quantitative (1)H NMR.

Keywords: Countercurrent separation; Generally useful estimate of solvent systems; Ginkgo biloba; Ginkgotoxin isomers; Quantitative (1)H NMR; Thin layer chromatography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chloroform / chemistry
  • Countercurrent Distribution
  • Methanol / chemistry
  • Pyridoxine / analogs & derivatives*
  • Pyridoxine / isolation & purification
  • Solvents / chemistry*
  • Stereoisomerism
  • Water / chemistry

Substances

  • 4'-O-methylpyridoxine
  • Solvents
  • Water
  • Chloroform
  • Pyridoxine
  • Methanol