Histone H2AX and the small RNA pathway modulate both non-homologous end-joining and homologous recombination in plants

Yiping Qi; Yong Zhang; Joshua A Baller; Daniel F Voytas

doi:10.1016/j.mrfmmm.2015.12.002

Histone H2AX and the small RNA pathway modulate both non-homologous end-joining and homologous recombination in plants

Mutat Res. 2016 Jan:783:9-14. doi: 10.1016/j.mrfmmm.2015.12.002. Epub 2015 Dec 4.

Authors

Yiping Qi¹, Yong Zhang², Joshua A Baller³, Daniel F Voytas³

Affiliations

¹ Department of Biology, East Carolina University, Greenville, NC 27858, USA. Electronic address: [email protected].
² Department of Genetics, Cell Biology & Development and Center for Genome Engineering, University of MN, Minneapolis, MN 55455, USA; Department of Biotechnology, School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
³ Department of Genetics, Cell Biology & Development and Center for Genome Engineering, University of MN, Minneapolis, MN 55455, USA.

PMID: 26687994
DOI: 10.1016/j.mrfmmm.2015.12.002

Abstract

Using a zinc finger nuclease (ZFN) that creates a site-specific DNA double-strand break (DSB) at the endogenous ADH1 locus, we provide genetic evidence that histone H2AX and DSB-induced small RNAs modulate DNA repair by both non-homologous end-joining (NHEJ) and homologous recombination (HR) in the model plant Arabidopsis thaliana.

Keywords: Arabidopsis thaliana; DNA double-strand break (DSB) repair; H2AX; Small RNA; Zinc finger nuclease (ZFN).

Published by Elsevier B.V.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / genetics*
Arabidopsis / metabolism
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism
DNA Breaks, Double-Stranded
DNA End-Joining Repair*
DNA Repair
Histones / genetics
Histones / metabolism*
Homologous Recombination*
Mutation
Protoplasts / physiology
RNA, Plant / metabolism*

Substances

Arabidopsis Proteins
Histones
RNA, Plant