A Novel Radiotracer for Imaging Monoacylglycerol Lipase in the Brain Using Positron Emission Tomography

ACS Chem Neurosci. 2016 Apr 20;7(4):484-9. doi: 10.1021/acschemneuro.5b00293. Epub 2016 Jan 6.

Abstract

Monoacylglycerol lipase (MAGL) is a serine hydrolase that hydrolyzes monoacylglycerols to glycerol and fatty acid and plays an important role in neuroinflammation. MAGL inhibitors are a class of molecules with therapeutic potential for human diseases of the central nervous system (CNS), in areas such as pain and inflammation, immunological disorders, and neurological and psychiatric conditions. Development of a noninvasive imaging probe would elucidate the distribution and functional roles of MAGL in the brain and accelerate medical research and drug discovery in this domain. Herein, we describe the synthesis and pilot rodent imaging of a novel MAGL imaging agent, [(11)C]SAR127303. Our imaging results demonstrate the high specificity, good selectivity, and appropriate kinetics and distribution of [(11)C]SAR127303, validating its utility for imaging MAGL in the brain. Our findings support the translational potential for human CNS MAGL imaging.

Keywords: CNS; Monoacylglycerol lipase; PET; brain; radiotracer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetamides / pharmacology
  • Animals
  • Brain / diagnostic imaging*
  • Brain / enzymology
  • Carbamates / chemical synthesis
  • Carbamates / pharmacokinetics
  • Carbon Isotopes / pharmacokinetics
  • Enzyme Inhibitors / chemical synthesis
  • Enzyme Inhibitors / pharmacokinetics
  • Kinetics
  • Male
  • Monoacylglycerol Lipases / metabolism*
  • Pilot Projects
  • Positron-Emission Tomography*
  • Rats
  • Rats, Sprague-Dawley
  • Sulfonamides / chemical synthesis
  • Sulfonamides / pharmacokinetics
  • Tomography Scanners, X-Ray Computed

Substances

  • 4-chlorobenzenesulfonylglycolic acid amide
  • Acetamides
  • Carbamates
  • Carbon Isotopes
  • Enzyme Inhibitors
  • SAR127303
  • Sulfonamides
  • Monoacylglycerol Lipases