Zebrafish Whole-Mount In Situ Hybridization Followed by Sectioning

Canan Doganli; Jens Randel Nyengaard; Karin Lykke-Hartmann

doi:10.1007/978-1-4939-3179-8_31

Zebrafish Whole-Mount In Situ Hybridization Followed by Sectioning

Methods Mol Biol. 2016:1377:353-63. doi: 10.1007/978-1-4939-3179-8_31.

Authors

Canan Doganli¹, Jens Randel Nyengaard², Karin Lykke-Hartmann^{3

4}

Affiliations

¹ Smith Cardiovascular Research Institute, University of California, San Francisco, CA, 94158-9001, USA.
² Stereology and Electron Microscopy Laboratory, Centre for Stochastic Geometry and Advanced Bioimaging, Aarhus University Hospital, Aarhus University, Aarhus C, Denmark.
³ Department of Biomedicine and Centre for Membrane Pumps in Cells and Disease - PUMPKIN, Danish National Research Foundation, Aarhus University, Aarhus C, Denmark. [email protected].
⁴ Aarhus Institute of Advanced Studies (AIAS), Aarhus University, Aarhus C, Denmark. [email protected].

PMID: 26695046
DOI: 10.1007/978-1-4939-3179-8_31

Abstract

In situ hybridization is a powerful technique used for locating specific nucleic acid targets within morphologically preserved tissues and cell preparations. A labeled RNA or DNA probe hybridizes to its complementary mRNA or DNA sequence within a sample. Here, we describe RNA in situ hybridization protocol for whole-mount zebrafish embryos.

Keywords: DIG-labeled RNA antisense probe; In situ hybridization; NBT/BCIP staining; Pre-hybridization; Sectioning; Zebrafish.

MeSH terms

Animals
Embryo, Nonmammalian / metabolism
Female
In Situ Hybridization / methods*
RNA Probes / genetics
Staining and Labeling / methods*
Zebrafish / embryology*

Substances

RNA Probes