Whole-Mount Immunohistochemistry for Anti-F59 in Zebrafish Embryos (1-5 Days Post Fertilization (dpf))

Canan Doganli; Lucas Bukata; Karin Lykke-Hartmann

doi:10.1007/978-1-4939-3179-8_32

Whole-Mount Immunohistochemistry for Anti-F59 in Zebrafish Embryos (1-5 Days Post Fertilization (dpf))

Methods Mol Biol. 2016:1377:365-9. doi: 10.1007/978-1-4939-3179-8_32.

Authors

Canan Doganli¹, Lucas Bukata², Karin Lykke-Hartmann^{3

4

5}

Affiliations

¹ Smith Cardiovascular Research Institute, University of California, San Francisco, USA.
² Sanford-Burnham Medical Research Institute, La Jolla, USA.
³ Department of Biomedicine and Centre for Membrane Pumps in Cells and Disease - PUMPKIN, Danish National Research Foundation, Aarhus University, Aarhus C, Denmark. [email protected].
⁴ Department of Molecular Biology and Genetics, Aarhus University, Aarhus C, Denmark. [email protected].
⁵ Aarhus Institute of Advanced Studies (AIAS), Aarhus University, Aarhus C, Denmark. [email protected].

PMID: 26695047
DOI: 10.1007/978-1-4939-3179-8_32

Abstract

Immunohistochemistry (IHC) is a powerful method to determine localization of tissue components by the interaction of target antigens with labeled antibodies. Here we describe an IHC protocol for localizing the myosin heavy chain of zebrafish embryos at 1-2 and 3-5 days post fertilization (dpf).

Keywords: F59; Immunohistochemistry; Myosin heavy chain; Zebrafish.

MeSH terms

Animals
Embryo, Nonmammalian / metabolism*
Embryo, Nonmammalian / physiology
Fertilization*
Gene Knockdown Techniques
Immunohistochemistry / methods*
Isoenzymes / deficiency
Isoenzymes / genetics
Mutation
Myosin Heavy Chains / metabolism
Sodium-Potassium-Exchanging ATPase / deficiency
Sodium-Potassium-Exchanging ATPase / genetics
Staining and Labeling
Time Factors
Zebrafish / embryology*

Substances

Isoenzymes
Myosin Heavy Chains
Sodium-Potassium-Exchanging ATPase