A sequence-independent single-primer amplification method and a modified enterovirus VP1 gene typing primer were used for identification of echovirus 19 and enterovirus 101, which remained undiagnosed by standard enterovirus molecular typing methods. Six different serotypes were identified during this study, with the predominance of ECV 19 (n = 20) followed by echovirus 21 (n = 3), EV 69 and EV 101 (n = 2 each), coxsackievirus B5 and ECV 27 (n = 1 each). To our knowledge, this is the first report of enteroviruses 69 and 101 in encephalitis cases in India.
Keywords: 5′UTR; Echovirus 19; Encephalitis; Enterovirus 101; Molecular typing; Phylogenetic analysis; SISPA; VP1.