Comparison of Multilocus Sequence Typing and the Xpert C. difficile/Epi Assay for Identification of Clostridium difficile 027/NAP1/BI

J Clin Microbiol. 2016 Mar;54(3):775-8. doi: 10.1128/JCM.03075-15. Epub 2015 Dec 23.

Abstract

Clostridium difficile 027/NAP1/BI is the most common C. difficile strain in the United States. The Xpert C. difficile/Epi assay allows rapid, presumptive identification of C. difficile NAP1. We compared Xpert C. difficile/Epi to multilocus sequence typing for identification of C. difficile NAP1 and found "very good" agreement at 97.9% (κ = 0.86; 95% confidence interval, 0.80 to 0.91).

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Clostridioides difficile / classification*
  • Clostridioides difficile / genetics*
  • Clostridium Infections / diagnosis
  • Clostridium Infections / epidemiology
  • Clostridium Infections / microbiology
  • Humans
  • Multilocus Sequence Typing*
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • RNA, Ribosomal, 23S / genetics

Substances

  • RNA, Ribosomal, 16S
  • RNA, Ribosomal, 23S