Principles of cryo-EM single-particle image processing

Fred J Sigworth

doi:10.1093/jmicro/dfv370

Principles of cryo-EM single-particle image processing

Microscopy (Oxf). 2016 Feb;65(1):57-67. doi: 10.1093/jmicro/dfv370. Epub 2015 Dec 24.

Author

Fred J Sigworth¹

Affiliation

¹ Department of Cellular and Molecular Physiology, Yale University, 333 Cedar Street, New Haven, CT 06520, USA Department of Molecular Biophysics and Biochemistry, Yale University, 333 Cedar Street, New Haven, CT 06520, USA [email protected].

Abstract

Single-particle reconstruction is the process by which 3D density maps are obtained from a set of low-dose cryo-EM images of individual macromolecules. This review considers the fundamental principles of this process and the steps in the overall workflow for single-particle image processing. Also considered are the limits that image signal-to-noise ratio places on resolution and the distinguishing of heterogeneous particle populations.

Keywords: 3D reconstruction; SNR; noise.

Publication types

Research Support, N.I.H., Extramural
Review

MeSH terms

Cryoelectron Microscopy / methods*
Imaging, Three-Dimensional / methods*
Macromolecular Substances / analysis*
Protein Conformation
Signal-To-Noise Ratio

Substances

Macromolecular Substances

Abstract

Publication types

MeSH terms

Substances

Grants and funding