In vivo depletion and genetic targeting of mouse intestinal CX3CR1(+) mononuclear phagocytes

Balázs Koscsó; Kavitha Gowda; Milena Bogunovic

doi:10.1016/j.jim.2015.12.009

In vivo depletion and genetic targeting of mouse intestinal CX3CR1(+) mononuclear phagocytes

J Immunol Methods. 2016 May:432:13-23. doi: 10.1016/j.jim.2015.12.009. Epub 2015 Dec 17.

Authors

Balázs Koscsó¹, Kavitha Gowda¹, Milena Bogunovic²

Affiliations

¹ Department of Microbiology and Immunology, Penn State University College of Medicine and Milton Hershey Medical Center, Hershey, PA 17033, USA.
² Department of Microbiology and Immunology, Penn State University College of Medicine and Milton Hershey Medical Center, Hershey, PA 17033, USA. Electronic address: [email protected].

PMID: 26705686
DOI: 10.1016/j.jim.2015.12.009

Abstract

Mononuclear phagocytes (MPs) are an essential component of the intestinal immune system. They are comprised of a few dendritic cell and macrophage subsets, all with the common ability to sample extracellular milieu and to discriminate between dangerous and innocuous signals. Despite the commonality, each MP subset acquires distinct developmental pathways and unique functions, likely to fulfill needs of the tissue in which they reside. Some MP subsets develop from monocytes and are distinguished by their expression of CX3C-chemokine receptor 1 (CX3CR1). This manuscript summarizes our expertise in vivo targeting of intestinal CX3CR1(+) MP subsets. The described tools might be useful for studies of CX3CR1(+) MP function in various murine experimental models, particularly under non-inflammatory conditions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / pharmacology
Biomarkers / metabolism
CX3C Chemokine Receptor 1
Cell Lineage
Dendritic Cells / drug effects
Dendritic Cells / immunology
Dendritic Cells / metabolism*
Down-Regulation
Gene Targeting / methods*
Genotype
Hybridomas
Immunity, Mucosal*
Immunophenotyping
Integrases / genetics
Intestinal Mucosa / metabolism*
Intestines / drug effects
Intestines / immunology
Macrophages / drug effects
Macrophages / immunology
Macrophages / metabolism*
Mice, Inbred C57BL
Mice, Knockout
Muramidase / genetics
Muramidase / immunology
Muramidase / metabolism
Phenotype
Promoter Regions, Genetic
Receptors, Chemokine / deficiency*
Receptors, Chemokine / genetics
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / antagonists & inhibitors
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / genetics
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / immunology
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / metabolism

Substances

Antibodies, Monoclonal
Biomarkers
CX3C Chemokine Receptor 1
Csf1r protein, mouse
Cx3cr1 protein, mouse
Receptors, Chemokine
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
Cre recombinase
Integrases
Muramidase