A simple method of bone marrow cryopreservation

Folia Histochem Cytobiol. 1989;27(2):67-71.

Abstract

Mouse bone marrow cells (BMC) were frozen by the simple two step method and stored in liquid nitrogen. The reconstitutive potency of fresh and frozen BMC were compared. BMC-fresh and BMC-frozen had a similar potential of colony formation in spleen and reconstitution of lethally total body irradiated (TBI) mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells*
  • Bone Marrow Transplantation
  • Colony-Forming Units Assay
  • Freezing
  • Mice
  • Tissue Preservation / methods*
  • Whole-Body Irradiation