ActivinB Is Induced in Insulinoma To Promote Tumor Plasticity through a β-Cell-Induced Dedifferentiation

Mol Cell Biol. 2015 Dec 28;36(5):756-64. doi: 10.1128/MCB.00930-15.

Abstract

Loss of pancreatic β-cell maturity occurs in diabetes and insulinomas. Although both physiological and pathological stresses are known to promote β-cell dedifferentiation, little is known about the molecules involved in this process. Here we demonstrate that activinB, a transforming growth factor β (TGF-β)-related ligand, is upregulated during tumorigenesis and drives the loss of insulin expression and β-cell maturity in a mouse insulinoma model. Our data further identify Pax4 as a previously unknown activinB target and potent contributor to the observed β-cell dedifferentiation. More importantly, using compound mutant mice, we found that deleting activinB expression abolishes tumor β-cell dedifferentiation and, surprisingly, increases survival without significantly affecting tumor growth. Hence, this work reveals an unexpected role for activinB in the loss of β-cell maturity, islet plasticity, and progression of insulinoma through its participation in β-cell dedifferentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activins / genetics
  • Activins / metabolism*
  • Animals
  • Cell Dedifferentiation*
  • Gene Expression Regulation, Neoplastic
  • Insulin / genetics
  • Insulin-Secreting Cells / cytology
  • Insulin-Secreting Cells / metabolism
  • Insulin-Secreting Cells / pathology*
  • Insulinoma / genetics
  • Insulinoma / metabolism
  • Insulinoma / pathology*
  • Mice, Inbred C57BL
  • Pancreas / metabolism
  • Pancreas / pathology*
  • Pancreatic Neoplasms / genetics
  • Pancreatic Neoplasms / metabolism
  • Pancreatic Neoplasms / pathology*
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism

Substances

  • Insulin
  • Men1 protein, mouse
  • Proto-Oncogene Proteins
  • activin B
  • Activins

Grants and funding

Olov Andersson thanks the Ragnar Söderbergs foundation, Swedish Research Council and Strategic Research Initiative in Stem Cells and Regenerative Medicine, for support.