Comparison of 2 chromogenic media for the detection of extended-spectrum β-lactamase producing Enterobacteriaceae stool carriage in nursing home residents

Diagn Microbiol Infect Dis. 2016 Mar;84(3):181-3. doi: 10.1016/j.diagmicrobio.2015.11.008. Epub 2015 Nov 11.

Abstract

ChromID ESBL agar and Brilliance ESBL agar were compared for the isolation of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae from 298 stools. These had comparable sensitivity and selectivity for the 116 positive samples. Pre-enrichment with cefpodoxime and extending incubation to 48 hours after direct plating both significantly increased sensitivity but reduced selectivity of both agars.

Keywords: Chromogenic agar; Detection; Enterobacteriaceae; Extended-spectrum β-lactamase; Selectivity; Sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Carrier State*
  • Cefpodoxime
  • Ceftizoxime / analogs & derivatives
  • Ceftizoxime / pharmacology
  • Disk Diffusion Antimicrobial Tests / methods
  • Enterobacteriaceae / classification
  • Enterobacteriaceae / drug effects
  • Enterobacteriaceae / enzymology*
  • Enterobacteriaceae / genetics
  • Enterobacteriaceae Infections / diagnosis*
  • Enterobacteriaceae Infections / microbiology*
  • Feces / microbiology*
  • Humans
  • Nursing Homes*
  • Sensitivity and Specificity
  • beta-Lactamases / biosynthesis*
  • beta-Lactamases / genetics

Substances

  • Anti-Bacterial Agents
  • Ceftizoxime
  • beta-Lactamases