A Recombinant Adenovirus Expressing Ovine Interferon Tau Prevents Influenza Virus-Induced Lethality in Mice

V Martín; E Pascual; M Avia; G Rangel; A de Molina; A Alejo; N Sevilla

doi:10.1128/JVI.03258-15

A Recombinant Adenovirus Expressing Ovine Interferon Tau Prevents Influenza Virus-Induced Lethality in Mice

J Virol. 2016 Jan 6;90(7):3783-8. doi: 10.1128/JVI.03258-15.

Authors

V Martín¹, E Pascual², M Avia², G Rangel², A de Molina³, A Alejo², N Sevilla²

Affiliations

¹ Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Valdeolmos, Madrid, Spain [email protected].
² Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Valdeolmos, Madrid, Spain.
³ Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain.

Abstract

Ovine interferon tau (IFN-τ) is a unique type I interferon with low toxicity and a broad host range in vivo. We report the generation of a nonreplicative recombinant adenovirus expressing biologically active IFN-τ. Using the B6.A2G-Mx1 mouse model, we showed that single-dose intranasal administration of recombinant Ad5-IFN-τ can effectively prevent lethality and disease induced by highly virulent hv-PR8 influenza virus by activating the interferon response and preventing viral replication.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenoviridae / genetics*
Animals
Disease Models, Animal
Genetic Therapy / methods
Genetic Vectors*
Interferon Type I / biosynthesis*
Interferon Type I / genetics
Mice
Orthomyxoviridae / immunology*
Orthomyxoviridae Infections / immunology
Orthomyxoviridae Infections / prevention & control*
Pregnancy Proteins / biosynthesis*
Pregnancy Proteins / genetics
Recombinant Proteins / biosynthesis*
Recombinant Proteins / genetics
Survival Analysis
Treatment Outcome

Substances

Interferon Type I
Pregnancy Proteins
Recombinant Proteins
interferon tau