Abstract
We comprehensively assessed the contribution of the Shine-Dalgarno sequence to protein expression and used the data to develop EMOPEC (Empirical Model and Oligos for Protein Expression Changes; http://emopec.biosustain.dtu.dk). EMOPEC is a free tool that makes it possible to modulate the expression level of any Escherichia coli gene by changing only a few bases. Measured protein levels for 91% of our designed sequences were within twofold of the desired target level.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Algorithms
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Cloning, Molecular
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Escherichia coli / genetics*
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Escherichia coli Proteins / genetics*
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Peptide Chain Initiation, Translational / genetics*
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Promoter Regions, Genetic / genetics
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Protein Engineering / methods*
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RNA, Bacterial / genetics*
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RNA, Messenger / genetics
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RNA, Ribosomal, 16S / genetics
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Sequence Homology, Nucleic Acid
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Software*
Substances
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Escherichia coli Proteins
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RNA, Bacterial
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RNA, Messenger
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RNA, Ribosomal, 16S