Impaired mitochondrial biogenesis is a common feature to myocardial hypertrophy and end-stage ischemic heart failure

Cardiovasc Pathol. 2016 Mar-Apr;25(2):103-12. doi: 10.1016/j.carpath.2015.09.009. Epub 2015 Sep 30.

Abstract

Mitochondrial (mt) DNA depletion and oxidative mtDNA damage have been implicated in the process of pathological cardiac remodeling. Whether these features are present in the early phase of maladaptive cardiac remodeling, that is, during compensated cardiac hypertrophy, is still unknown. We compared the morphologic and molecular features of mt biogenesis and markers of oxidative stress in human heart from adult subjects with compensated hypertrophic cardiomyopathy and heart failure. We have shown that mtDNA depletion is a constant feature of both conditions. A quantitative loss of mtDNA content was associated with significant down-regulation of selected modulators of mt biogenesis and decreased expression of proteins involved in mtDNA maintenance. Interestingly, mtDNA depletion characterized also the end-stage phase of cardiomyopathies due to a primary mtDNA defect. Oxidative stress damage was detected only in failing myocardium.

Keywords: Cardiac remodeling; Mitochondrial biogenesis; Mitochondrial cardiomyopathy; Myocardial hypertrophy; Oxidative stress; mtDNA depletion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Blotting, Western
  • DNA, Mitochondrial / metabolism
  • Female
  • Heart Failure / etiology
  • Heart Failure / pathology*
  • Humans
  • Hypertrophy, Left Ventricular / pathology*
  • Laser Capture Microdissection
  • Male
  • Microscopy, Electron, Transmission
  • Middle Aged
  • Myocardial Ischemia / complications*
  • Organelle Biogenesis*
  • Oxidative Stress / physiology*
  • Real-Time Polymerase Chain Reaction
  • Ventricular Remodeling / physiology*

Substances

  • DNA, Mitochondrial