Genome-wide association studies based on sequence-derived genotypes reveal new QTL associated with conformation and performance traits in the Franches-Montagnes horse breed

Anim Genet. 2016 Apr;47(2):227-9. doi: 10.1111/age.12406. Epub 2016 Jan 14.

Abstract

To identify novel quantitative trait loci (QTL) within horses, we performed genome-wide association studies (GWAS) based on sequence-level genotypes for conformation and performance traits in the Franches-Montagnes (FM) horse breed. Sequence-level genotypes of FM horses were derived by re-sequencing 30 key founders and imputing 50K data of genotyped horses. In total, we included 1077 FM horses genotyped for ~4 million SNPs and their respective de-regressed breeding values of the traits in the analysis. Based on this dataset, we identified a total of 14 QTL associated with 18 conformation traits and one performance trait. Therefore, our results suggest that the application of sequence-derived genotypes increases the power to identify novel QTL which were not identified previously based on 50K SNP chip data.

Keywords: GWAS; NGS; imputation.

MeSH terms

  • Animals
  • Body Height / genetics
  • Breeding*
  • Gait / genetics
  • Genetic Association Studies
  • Genotype
  • Horses / genetics*
  • Humans
  • Models, Genetic
  • Polymorphism, Single Nucleotide
  • Quantitative Trait Loci*
  • Sequence Analysis, DNA