Cellular prion protein regulates invasion and migration of breast cancer cells through MMP-9 activity

Biochem Biophys Res Commun. 2016 Jan 29;470(1):213-219. doi: 10.1016/j.bbrc.2016.01.038. Epub 2016 Jan 9.

Abstract

Function of cellular prion protein (PrP(c)) in cancer progression has not been elucidated yet. Ectopic expression of PrP(c) increases the invasion and migration of breast cancer cell line, MCF-7 cells. Overexpressed PrP(c) increases matrix metalloprotease-9 (MMP-9) expression by enhancing association of NF-κB in promoter of MMP-9 gene and ERK signaling in MCF-7 cells. Whereas, silencing of PrP(c) by siRNA suppresses ERK activation and MMP-9 expression resulting the down-regulation of MD-MB231 cell migration and invasion. Overall, these results suggest that PrP(c) contribute the breast cancer invasion and migration via MMP-9.

Keywords: Breast cancer; Invasion; Matrix metalloprotease-9; Migration; Prion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / pathology*
  • Cell Movement
  • Enzyme Activation
  • Humans
  • MAP Kinase Signaling System*
  • MCF-7 Cells
  • Matrix Metalloproteinase 9 / metabolism*
  • NF-kappa B / metabolism*
  • Neoplasm Invasiveness
  • PrPC Proteins / metabolism*

Substances

  • NF-kappa B
  • PrPC Proteins
  • Matrix Metalloproteinase 9