Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India

Simerpreet Kaur; Vibhuti Rana; Pooja Singh; Garima Trivedi; Shashi Anand; Amanpreet Kaur; Pawan Gupta; Amita Jain; Charu Sharma

doi:10.1016/j.tube.2015.10.012

Novel mutations conferring resistance to kanamycin in Mycobacterium tuberculosis clinical isolates from Northern India

Tuberculosis (Edinb). 2016 Jan:96:96-101. doi: 10.1016/j.tube.2015.10.012. Epub 2015 Nov 28.

Authors

Simerpreet Kaur¹, Vibhuti Rana¹, Pooja Singh², Garima Trivedi¹, Shashi Anand¹, Amanpreet Kaur¹, Pawan Gupta¹, Amita Jain², Charu Sharma³

Affiliations

¹ CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
² Department of Microbiology, King George Medical University, Lucknow 226003, Uttar Pradesh, India.
³ CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India. Electronic address: [email protected].

PMID: 26786660
DOI: 10.1016/j.tube.2015.10.012

Abstract

Twenty-nine Kanamycin resistant clinical isolates of Mycobacterium tuberculosis from Northern India were screened to evaluate genetic mutations in rrs gene, eis gene with its promoter, and whiB7 gene along with its 5'UTR. 14 strains (~48.0%) collectively exhibited mutations in rrs, eis or whiB7 target regions. While the highest frequency of mutations was found in rrs gene, eis and whiB7 loci displayed novel mutations. The novel mutations displayed by eis and whiB7 loci were found to be associated specifically with the Kanamycin resistance as none of the twenty nine Kanamycin sensitive strains harbor them. The inclusion of novel mutations of eis and whiB7 loci will be useful in improving the specificity of future diagnostics.

Keywords: Aminoglycosides; MDR-TB; Molecular characterization; XDR-TB.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

5' Untranslated Regions
Acetyltransferases
Antigens, Bacterial / genetics
Antitubercular Agents / therapeutic use*
Bacterial Proteins / genetics
DNA Mutational Analysis
DNA, Bacterial / genetics*
Drug Resistance, Multiple, Bacterial / genetics*
Genotype
Humans
India
Kanamycin / therapeutic use*
Microbial Sensitivity Tests
Mutation*
Mycobacterium tuberculosis / drug effects
Mycobacterium tuberculosis / genetics*
Phenotype
Polymerase Chain Reaction
Predictive Value of Tests
Promoter Regions, Genetic
Tuberculosis, Multidrug-Resistant / diagnosis
Tuberculosis, Multidrug-Resistant / drug therapy
Tuberculosis, Multidrug-Resistant / microbiology*

Substances

5' Untranslated Regions
Antigens, Bacterial
Antitubercular Agents
Bacterial Proteins
DNA, Bacterial
Kanamycin
Acetyltransferases
Eis protein, Mycobacterium tuberculosis