Ultrasensitive In Situ Fluorescence Analysis using Modulated Fluorescence Interference Contrast at Nanostructured Polymer Surfaces

Adv Mater. 2016 Mar 23;28(12):2367-73. doi: 10.1002/adma.201505197. Epub 2016 Jan 25.

Abstract

The precise modulation of fluorescence interference contrast is achieved by introducing a nanoscopically engineered spacer layer prepared by chemical vapor deposition (CVD) of functional polymers. These novel imaging substrates are chemically identical throughout their entire detection area, yet present patterns of nanoscale thickness. A protein binding cascade is studied in real time and in the presence of high background noise.

Keywords: CVD; fluorescence interference contrast; lock-in analysis; nanostructured surfaces; protein adsorption; reactive polymers.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adsorption
  • Animals
  • Cattle
  • Fluorescein / chemistry
  • Gold / chemistry
  • Microscopy, Fluorescence
  • Nanostructures / chemistry*
  • Photoelectron Spectroscopy
  • Polymers / chemistry*
  • Serum Albumin, Bovine / chemistry
  • Surface Properties

Substances

  • Polymers
  • Serum Albumin, Bovine
  • Gold
  • Fluorescein