Histopathological and Biochemical Effects of Ecballium elaterium on Sepsis-Induced Lung Injury

J Invest Surg. 2016 Oct;29(5):302-8. doi: 10.3109/08941939.2015.1111474. Epub 2016 Jan 29.

Abstract

Purpose: The aim of this study was to investigate the role of Ecballium elaterium (EE) on sepsis-induced lung injury.

Materials and methods: A total of 30 male rats were divided into three groups as follows: control, sepsis, and treatment groups (sepsis + EE) with each group containing 10 rats. A rat model of sepsis induced by cecal ligation and puncture (CLP) was used. In the treatment group, rats were injected intraperitoneally with 2.5 mg/kg EE after CLP. Interleukin-6 (IL-6), tumor necrosis factor (TNF)-α, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) values after a 24-hr period were measured via cardiac puncture. Animals were harvested after the procedure and biochemical analysis was done and histopathological changes of the tissue sections of lungs were examined thereafter.

Results: A statistically significant decrease was observed in the IL-6 (p < .05), TNF-α (p < .05), and TOS (p < .01) levels in the sera of the treatment group compared to those of the sepsis group. Following the treatment, the TOS (p = .01) and OSI (p < .05) levels in the lung tissue of rats indicated a statistically significant decrease compared to those of the sepsis group. The histopathological follow-up undertaken after the administration of the EE treatment to septic rats showed significantly lower values of alveolar wall thickness (p < .001), interstitial edema (p = .018), and neutrophil infiltration (p = .047).

Conclusion: EE treatment may have beneficial effects on sepsis-induced lung injury, and therefore has potential for clinical use.

Keywords: Ecballium elaterium; lung; rat; sepsis; total antioxidant status; total oxidant status.

MeSH terms

  • Acute Lung Injury / drug therapy*
  • Acute Lung Injury / etiology*
  • Acute Lung Injury / metabolism
  • Animals
  • Antioxidants / metabolism
  • Cucurbitaceae*
  • Disease Models, Animal
  • Interleukin-6 / blood
  • Interleukin-6 / metabolism
  • Lung / drug effects
  • Lung / metabolism
  • Lung / pathology
  • Male
  • Oxidative Stress / drug effects
  • Phytotherapy*
  • Rats
  • Rats, Wistar
  • Sepsis / complications*
  • Tumor Necrosis Factor-alpha / blood
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Antioxidants
  • Interleukin-6
  • Tumor Necrosis Factor-alpha